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mouse primary antibodies gfap  (Proteintech)


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    Proteintech mouse primary antibodies gfap
    Mouse Primary Antibodies Gfap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 602 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+primary+antibodies+gfap/GFAP+Polyclonal+antibody/pm41348686-111-12-17
    Average 96 stars, based on 602 article reviews
    mouse primary antibodies gfap - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Co-regulation of microglial subgroups in Alzheimer’s amyloid pathology: Implications for diagnosis and drug development
    Article Snippet: Equal amounts of protein were separated on 12% SDS-PAGE SurePAGETM (Genscript, M00669) and transferred onto polyvinylidene fluoride membranes (Merck, 3010040001). .. Then the membranes were blocked and incubated overnight at 4 °C with mouse primary antibodies GFAP (1:5000, Proteintech, 16825-1-AP), AIF1 (IBA1) (1:1000, Cell Signaling Technology, 17198), TSPO (1:20000, Abcam, ab109497), and β-Actin (1:5000, Proteintech, 81115-1-RR). .. After washing, the membranes were incubated with secondary anti-rabbit HRP-conjugated antibodies (1:10000, Sangon, D110058-0001) and visualized using the enhanced chemiluminescent HRP substrate (Beyotime, P0018FS).

    Article Title: Co-regulation of microglial subgroups in Alzheimer's amyloid pathology: Implications for diagnosis and drug development.
    Article Snippet: Equal amounts of protein were separated on 12% SDS-PAGE SurePAGETM (Genscript, M00669) and transferred onto polyvinylidene fluoride membranes (Merck, 3010040001). .. Then the membranes were blocked and incubated overnight at 4 °C with mouse primary antibodies GFAP (1:5000, Proteintech, 16825-1-AP), AIF1 (IBA1) (1:1000, Cell Signaling Technology, 17198), TSPO (1:20000, Abcam, ab109497), and β-Actin (1:5000, Proteintech, 81115-1-RR). .. After washing, the membranes were incubated with secondary anti-rabbit HRP-conjugated antibodies (1:10000, Sangon, D110058-0001) and visualized using the enhanced chemiluminescent HRP substrate (Beyotime, P0018FS).



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    Indicaxanthin ameliorates brain inflammation induced by HFD. (A) Transcription levels of iNOS, TNF-α, and IL-6 determined by real-time PCR in the brain. (B) Brain expression of COX-2 and iNOS. (C) Densitometric analysis of COX-2 and iNOS protein levels normalized to β-actin levels. (D) Brain expression of cytosolic p65 and nuclear p65. (E) Densitometric analysis of cytosolic p65 protein levels normalized to β-actin levels and nuclear p65 levels normalized to laminin B levels. (F) Representative images of <t>GFAP-positive</t> cells (red) on the surface. Hoechst staining was used to label the nuclei (blue) (microscope magnification 10×); scale bars: 100 μm. (G) Percentage of GFAP-positive cells. (H, I) Plasma circulating levels of TNF-α (H) and IL-1β (I). Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; ### P < 0.001, vs. HFD-fed mice. COX-2: Cyclooxygenase-2; GFAP: glial <t>fibrillary</t> acidic protein; HFD: high-fat diet; IL-6: interleukin-6; Ind: indicaxanthin; iNOS: inducible nitric oxide synthase; PCR: polymerase chain reaction; STD: standard diet; TNF-α: tumor necrosis factor-α.
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    Indicaxanthin ameliorates brain inflammation induced by HFD. (A) Transcription levels of iNOS, TNF-α, and IL-6 determined by real-time PCR in the brain. (B) Brain expression of COX-2 and iNOS. (C) Densitometric analysis of COX-2 and iNOS protein levels normalized to β-actin levels. (D) Brain expression of cytosolic p65 and nuclear p65. (E) Densitometric analysis of cytosolic p65 protein levels normalized to β-actin levels and nuclear p65 levels normalized to laminin B levels. (F) Representative images of GFAP-positive cells (red) on the surface. Hoechst staining was used to label the nuclei (blue) (microscope magnification 10×); scale bars: 100 μm. (G) Percentage of GFAP-positive cells. (H, I) Plasma circulating levels of TNF-α (H) and IL-1β (I). Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; ### P < 0.001, vs. HFD-fed mice. COX-2: Cyclooxygenase-2; GFAP: glial fibrillary acidic protein; HFD: high-fat diet; IL-6: interleukin-6; Ind: indicaxanthin; iNOS: inducible nitric oxide synthase; PCR: polymerase chain reaction; STD: standard diet; TNF-α: tumor necrosis factor-α.

    Journal: Neural Regeneration Research

    Article Title: Positive impact of indicaxanthin from Opuntia ficus-indica fruit on high-fat diet–induced neuronal damage and gut microbiota dysbiosis

    doi: 10.4103/NRR.NRR-D-23-02039

    Figure Lengend Snippet: Indicaxanthin ameliorates brain inflammation induced by HFD. (A) Transcription levels of iNOS, TNF-α, and IL-6 determined by real-time PCR in the brain. (B) Brain expression of COX-2 and iNOS. (C) Densitometric analysis of COX-2 and iNOS protein levels normalized to β-actin levels. (D) Brain expression of cytosolic p65 and nuclear p65. (E) Densitometric analysis of cytosolic p65 protein levels normalized to β-actin levels and nuclear p65 levels normalized to laminin B levels. (F) Representative images of GFAP-positive cells (red) on the surface. Hoechst staining was used to label the nuclei (blue) (microscope magnification 10×); scale bars: 100 μm. (G) Percentage of GFAP-positive cells. (H, I) Plasma circulating levels of TNF-α (H) and IL-1β (I). Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; ### P < 0.001, vs. HFD-fed mice. COX-2: Cyclooxygenase-2; GFAP: glial fibrillary acidic protein; HFD: high-fat diet; IL-6: interleukin-6; Ind: indicaxanthin; iNOS: inducible nitric oxide synthase; PCR: polymerase chain reaction; STD: standard diet; TNF-α: tumor necrosis factor-α.

    Article Snippet: Next, the sections were incubated with a mouse primary antibody against glial fibrillary acidic protein (GFAP) (1:300, Cell Signaling Technology, Danvers, MA, USA, Cat# 3670, RRID: AB_10693476) at 4°C overnight and then with an anti-mouse IgG-Alexa Fluor 594 secondary antibody (goat, 1:300, Invitrogen, Cat# A-11005, RRID: AB_2534073) for 2 hours at room temperature.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Staining, Microscopy, Clinical Proteomics, Polymerase Chain Reaction